Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Alternative splicing results in multiple transcript variants. Many pseudogenes similar to this locus are present in the human genome. The regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, This protein contains a peptide that has antimicrobial activity against E. The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. GAPDH gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The molecular weight (MW) of GAPDH is approximately 37 kD. by using anti-GAPDH monoclonal antibody, Loading Control ( TA802519) Western blot analysis of extracts (35ug) from 9 different cell lines (HepG2: human HeLa: human SVT2: mouse A549: human COS7: monkey Jurkat: human MDCK: canine PC12: rat MCF7: human). View all GAPDH loading control antibodies. OriGene offers high quality ofĪnti-GAPDH TrueRAB™ antibody (Loading control). Protein detected by confirming that protein loading is the same across the gel. GAPDH antibody, is usually used as a loading control antibody for Western Blot to normalize the levels of Read this paper ' Housekeeping Gene Selection Advisory: GAPDH and Beta-actin'. Loading control in western blots and a positive control in qPCR experiments. Like beta-actin, GAPDH is a commonly used These functions are essential for basicĬell function, so GAPDH expression is stable and ubiquitous thus making it an ideal housekeeping gene. GAPDH, a multifunctional protein, not only catalyzes the oxidative phosphorylation of glyceraldehyde-3-phosphate during glycolysis, but also regulates multiple cellularįunctions including membrane fusion, transport, apoptosis, DNA replication and repair, and regulation of transcription and translation.
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